Bat coronavirus RS4081 Spike Trimer Protein (R653A, K954P, V955

The spike protein of bat coronavirus RS4081, a sarbecovirus related to SARS-CoV, mediates viral entry by facilitating attachment and membrane fusion. RS4081’s spike can support ACE2-independent, trypsin-dependent cell entry, highlighting alternative pathways for host infection and its potential relevance to zoonotic spillover and cross-species transmission studies.