Effect Diluent Medium, Animal-free

The Effect Diluent is an effective buffer for the dilution of serum, plasma, blood, stool or urine samples, as well as the dilution of primary and secondary antibodies. It efficiently minimizes cross-reactions and unspecific binding in immunoassays like ELISA, EIA, RIA, Western Blotting, Immuno-PCR, Protein arrays, multianalyte immunoassays and Immunohistochemistry.
The Effect Diluent is used alternatively to the standard sample or antibody dilution buffer:
In ELISA for the dilution of specimen and detection antibodies.
In Western Blotting for the dilution of primary and secondary antibodies.
In IHC for the dilution of primary and secondary antibodies.
In Protein arrays for the dilution of specimen and detection antibodies.
Three versions of the Effect Diluent are offered: Low, Medium and High for optimal discrimination between specific and unspecific reaction and for minimizing strong interference effects e.g. by RF (rheumatoid factors), HAMAs (human-a-mouse Abs) or by endogenous components that bind and mask the analyte.