pNPP ONE Substrate

pNPP (p-nitrophenylphosphate) is a chromogenic substrate for Alkaline Phosphatase (AP). In the presence of AP, pNPP is hydrolyzed rapidly to pnitrophenol and inorganic phosphate. Liberated pnitrophenol is seen as a clear yellow color that is measured at 405/620 nm. pNPP ONE liquid ready-to-use substrate is active and stable. PNPP ONE shows superior background characteristics due to its high temperature robustness compared to pNPP (cat. no. 4400).