How to use low volume non-contact dispensing for high-throughput next-generation-sequencing for Covid-19 surveillance
Next-generation sequencing (NGS) is a high-throughput methodology of sequencing. It’s compatible with a range of samples, including microbial genomes and transcriptomes.
Immediate drop-on-demand technology is an approach for optimizing noncontact liquid-handling tasks. The system uses eight individually controlled positive pressure channels to generate droplets from eight to fifty nanoliters.
This occurs from a small outlet at the bottom of each well. Each channel can generate up to a hundred droplets per second. With this approach, the technology enables a volume range from eight nanoliters to eighty microliters.
The non-contact dispensing reduces the number of pipette tips needed throughout the NGS library prep. The low-volume dispensing (dead volume <1 µL) provides significant value savings on reagents.
How a research group from the Karolinska Institute developed the COVseq method
A group from the Karolinska Institute performed a genomic sequencing study of the coronavirus. They conducted their study using a technique referred to as CUTseq. This is a useful technique for preparing multiplex DNA sequencing libraries from low-input samples.
They found that CUTseq is applicable for current pandemic genomic surveillance. The method is also adaptable to other pathogens like influenza viruses.
A cost analysis also showed how you can use COVseq based on CUTseq to sequence thousands of samples every week. The method allows you to sequence samples at less than $10 USD per sample. This includes both library preparation and sequencing costs
In this blog post, we describe how the Karolinska Institute research group of Nicola Crosetto, PhD and MD, used Cellinks Immediate Drop-on-demand Technology (I.DOT) to automate and optimize the COVseq workflow. This ensured high precision, speed, and significant reduction of liquid reagent volumes.
Conduct an affordable & high-throughput workflow and use it for mass-scale Coronavirus genomic surveillance
Since the identification of the Coronavirus, researchers globally have sequenced virus genomes. They even made their sequences available to the public.
The scientific movement caused by the SARS-CoV-2 enabled a phylogenetic reconstruction of viral spreading. It also enabled an evolution in different countries and continents.
This caused researchers to quickly identify any genomic variations of epidemiological concern.
Scientists are growing their interest in applying SARS-CoV-2 whole-genome sequencing (WGS). In their epidemiological surveillance, they track infections in hospitals and other communities. The purpose is to inform public health decisions.
Are you wasting money and reagents?
Rapid and cost-effective methods for sequencing are very beneficial. This way researchers can sequence hundreds or even thousands of samples per week.
That is why the team of Nicola Crosetto used a handy and adaptable method for preparing multiplex DNA sequencing libraries from low-input samples. They called this CUTseq.
They based their COVseq protocol on CUTseq. This allowed them to construct highly multiplexed sequencing libraries. They started with small volumes of purified RNA samples. They used the Cellink I.DOT, which reduced reagent volumes. It also reduced the cost per sample.
To get further details on the procedures, materials, and methods involved in this study, read the full article here.
How did they do it?
The Swedish research team was able to perform these experiments using innovative technology. They found the I.DOT to be versatile and easy to use.
Do you want to know more about how you can optimize your noncontact liquid-handling tasks?
Our Product Specialists are happy to help you with any of your inquiries. Contact us here.
If you want to know more about how you can benefit from the I.DOT in your laboratory, you can read about it here.