PentaBase is offering high quality standard DNA probes and primers but also improved probes and primers. PentaBase offers two different types of dual labeled probes for Real-Time PCR: Molecular Beacons/EasyBeacons™ and TaqMan®/HydrolEasy™ probes. Dual labeled probes normally contain a quencher at the 3′-end and a fluorophore at the 5′-end, but can also contain the fluorophore and/or quencher internally in the sequence.

 

HydrolEasy™ probes – PentaBase’s great alternative to TaqMan®MGB probes

HydrolEasy™ probes are hydrolysis probes with significantly better signal-to-noise ratio, a higher specificity and a higher sensitivity than conventional probes. When the HydrolEasy probe is intact the fluorophore and the quencher are, in contrast to “standard” TaqMan® probes, kept in close proximity by hydrophobic interactions between pentabases. During the amplification reaction the probe hybridizes to the target and exonuclease activity of the polymerase cleaves of the fluorophore from the probe whereby a strong fluorescence signal is generated. The figure below shows how a HydrolEasy™ probe degrades in the same way as normal TaqMan® probes do.

They convert your TaqMan®MGB designs and standard hydrolysis probe designs into HydrolEasy™ probes for free! Just an email with your sequences. It is no problem if you do not have a design already, just send an email with the details that you have. Please note that TaqMan MGB is a registered trademark of Life Technologies. Their probes do not comprise a minor groove binder, but instead they comprise several pentabases. The pentabases not only increase signal-to-noise ratios, but also increase affinity, specificity and sensitivity.

EasyBeacons™ – The easy to use and design alternative to Molecular Beacons

EasyBeacons™ are nuclease resistant probes developed by PentaBase™ to be used in 3-step Real-Time PCR and in situ hybridizations. Like Molecular Beacons EasyBeacons™ are quenched when the probes are unbound, but fluoresce at hybridization to a target sequence. However, unlike Molecular Beacons EasyBeacons™ do not require an internal stem to be quenched efficiently. The pentabases in EasyBeacons™ will, by having hydrofobic interactions with each other in the unbound probe, ensure that the quencher and fluorophore are kept in close proximity and hence resulting in an efficiently quenched probe with low background fluorescence.

Due to EasyBeacons™ nuclease resistance, they can also be used for end-point detection or verification of your Real-Time signal. This is a valuable feature, especially if you want to discriminate between almost similar genetic sequences like SNPs, alleles or if you want a quality control on your quantification data.

 

Do you want to test EasyBeacons™, they design them for you for free! Just send an email with your target sequences. It is no problem if you do not have a design already, just send an email with the details that you have.