We are now introducing the new indexed adapters for next generation sequencing on Illumina’s platform. Using outperforming technologies, they have made indexed adapters ready-to-use and ready-to-ship, for the sensitive sequencing of multiplexed samples.

The Figure above illustrate an indexed PentAdapter™. From the left top, there is a sequence that can bind to the flowcells used in Illumina’s sequence instruments like the MiSeq. Then an area for possible universal primer targeting. Following is a complementary region and finally a T-tail overhang. From the bottom left there is a binding site for universal priming. Then an index region. Following is a complementary region and finally a 5′-phosphorylation. The special PentaBase modifications are not shown in this illustration for proprietary reasons.
PentAdapters are robust, sensitive and work with a range of different DNA prep kits.

DNA analysis after preparation using different DNA preparation kits. Instructions from manufactures were followed. DNA purification on beads was used on all samples except lane 4 where gel based purification was performed. From left: Lane 1: DNA ladder. Lane 2: Illumina’s DNA prep + Competitor I’s Adapter. Lane 3: Illumina’s DNA prep + PentAdapter. Lane 4: Illumina’s DNA prep + Competitor I’s Adapter (size select on gel). Lane 5: Kapa Biosystem’s DNA prep + PentAdapter. Lane 6: NEB’s DNA prep + Custom adapter from PentaBase. Lane 7: NEB’s DNA prep + PentAdapter.

The adaptors are available in several different amounts, with flexible number of different indexes and can be send either lyophilised or in solution.