PCRBIO HiFi Polymerase was derived from Pfu DNA polymerase for its’ 3’-5’ exonuclease (proofreading) activity in PCR. Several proprietary point-mutations allow for significantly improved performance when compared with its native form.

Together with advanced buffer chemistry this enzyme brings robust performance to the world of high fidelity PCR. Enhanced DNA binding allows for improved processivity, increasing yield and shortening cycling times. The enhanced efficiency of PCRBio HiFi Polymerase minimises PCR inhibition, from impure samples such as colony PCR and direct PCR.


  • 50x higher fidelity than Taq DNA polymerase
  • Increased PCR success rates with amplicons up to 10kb
  • Advanced buffer chemistry including Mg and dNTPs
  • High yields under standard and fast PCR conditions
  • Efficient specific amplification from complex templates including GC rich and AT rich sequences