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Assessment of large DNA fragments size, quality and quantity

Reliable quality assessment of large DNA fragments/smears is essential in a number of downstream
applications, such as long read next generation sequencing, FFPE DNA analysis and DNA restriction
analysis. Traditionally, quality analysis of large size DNA fragments is performed by both agarose gel
electrophoresis and pulsed-field gel electrophoresis, both of which are time and labor consuming.

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Large Fragment Analysis Brochure

The Fragment Analyzer estimated sample size is compared to the expected size generated from pulsed-field gel electrophoresis. The sizing accuracy and precision was <10%. The concentration of the samples estimated by Fragment Analyzer was compared to the concentration values estimated by Qubit®. The quantitation accuracy was <15% and precision was <5%
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