crispr

Mutation analysis by capillary electrophoresis provides significant benefits over slab gel methods. The
Fragment Analyzer detects mutations generated from Non Homologous End Joining (NHEJ) and
Homology Directed Repair (HDR) modifications to targeted gene sequences. As a multichannelcrispr-cas9
capillary electrophoresis instrument that uses performance tested non-denaturing Gel Kits coupled with a sensitive intercalating dye, the Fragment Analyzer automates workflow, reduces hands on time and decreases time to results while providing high detection sensitivity and separation resolution. PROSize® integrated analytical software package accelerates detection of mutation events by efficiently displaying results.

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For gene editing experiments, the CRISPR-specific Gel Kit will show the mutation frequency of a pooled cell population, determine the presence/absence of a mutation from a single cell compared to its wild type complement, and under certain circumstances, assist in calling the zygosity of alleles. Additionally, researchers can determine the quality and quantity of isolated genomic DNA prior to amplification.

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They can measure amplicon post-PCR to assist in combiningFragment_analyzer
equimolar amounts of pre-heteroduplexed wild type and
experimental amplicon.
The Fragment Analyzer can be used in multiple ways related to
CRISPR/Cas9 mutagenesis including single guide RNA (sgRNA)
for cleavage efficiency, analyzing mutation events in cell lines or
pools of individual cells, plant cells and microbes. Furthermore,
time course studies or changes to cell lines during cell selective
passage can be monitored.